Figure 5: DXM inhibition of MAPK and NF-κB activation in BMDCs. Immature BMDCs were stimulated with 100 ng/mL LPS with or without DXM and lysed at the indicated time points. (a) The ERK, JNK, and p38 MAPK (native and phosphorylated) in whole cell lystes. (b) IκBα in whole-cell lystes and NF-κB p65 in nuclear extracts as mentioned were determined by Western blot with antibodies. β-actin and Lamin B loading controls are also shown to demonstrate relatively equal protein load across all lanes. The data are representative of three independent experiments showing similar results.