Modulatory Effect of 1,25-Dihydroxyvitamin D3 on IL1β-Induced RANKL, OPG, TNFα, and IL-6 Expression in Human Rheumatoid Synoviocyte MH7A
Effect of 1,25(OH)2D3 on ILβ-induced RANKL and OPG expression in MH7A and primary RA fibroblast-like synoviocyte. MH7A cells were stimulated with 20 ng/mL IL1β and then treated with different concentrations of 1,25(OH)2D3 (0.1 nM, 1 nM, 10 nM, and 100 nM) for 48 h.The effect of 1,25(OH)2D3 on RANKL and OPG expression in IL1β-induced MH7A (a) and RA-FLS (c) was analyzed by real-time PCR.The alteration of OPG/RANKL ratio in IL1β-induced MH7A (b) and RA-FLS (d) was analyzed (b). The data points shown are the mean ± SD for three independent experiments, each in triplicate. * compared to cells cultured with IL-1 alone.
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