Modulatory Effect of 1,25-Dihydroxyvitamin D3 on IL1β-Induced RANKL, OPG, TNFα, and IL-6 Expression in Human Rheumatoid Synoviocyte MH7A
Effect of 1,25(OH)2D3 on ILβ-induced TNFα and IL-6 production in MH7A. MH7A cells were stimulated with 20 ng/mL IL1 and then treated with different concentrations of 1,25(OH)2D3 (0.1 nM, 1 nM, 10 nM, and 100 nM) for 48 h. The effects of 1,25(OH)2D3 on ILβ-induced TNFα (a) and IL-6 (b) mRNA expression in MH7A was analyzed by real-time PCR, and the production of IL-6 in supernatants was shown in (c). The data shown are the mean ± SD for three independent experiments, each in triplicate. * compared to cells cultured with IL-1 alone. ** compared to cells cultured with IL-1 alone.
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