Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence
Figure 4
Study of fluorescence index as a function of dilution. Twenty ANA positive samples with different fluorescence patterns were diluted from 1 : 100 to 1 : 800. For each fluorescence pattern, four representative samples were diluted. For each sample, one curve represents the fluorescence index plotted as a function of dilution factor. SP: speckled, NU: nucleolar, H: homogenous, ND: nuclear dots, HSP: homogenous-speckled, and CE: centromeric.