Research Article

Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence

Figure 4

Study of fluorescence index as a function of dilution. Twenty ANA positive samples with different fluorescence patterns were diluted from 1 : 100 to 1 : 800. For each fluorescence pattern, four representative samples were diluted. For each sample, one curve represents the fluorescence index plotted as a function of dilution factor. SP: speckled, NU: nucleolar, H: homogenous, ND: nuclear dots, HSP: homogenous-speckled, and CE: centromeric.
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