Ex Vivo Restimulation of Human PBMC Expands a CD3⁺CD4⁻CD8⁻𝛾𝛿⁺ T Cell Population That Can Confound the Evaluation of CD4 and CD8 T Cell Responses to Vaccination

Figure 1

Characterisation of an IFN-𝛾-producing CD3⁺CD4⁻CD8⁻𝛾𝛿⁺ T cell-positive subject at day 7 after vaccination. PBMCs were expanded in vitro in the absence of exogenous influenza virus antigen, and the expression of CD4, CD8, 𝛼𝛽 TCR, CD161, V𝛼24, and 𝛾𝛿 TCR surface antigens was assessed by flow cytometry.