Ex Vivo Restimulation of Human PBMC Expands a CD3⁺CD4⁻CD8⁻𝛾𝛿⁺ T Cell Population That Can Confound the Evaluation of CD4 and CD8 T Cell Responses to Vaccination

Table 1

Number of subjects and percentage of IFN-𝛾-producing CD3⁺CD4⁻CD8⁻𝛾𝛿⁺ T-cell responders and nonresponders detected in each of the four study cohorts which comprised of various formulations of influenza and ISCOMATRIX adjuvant. The number of CD3⁺CD4⁻CD8⁻𝛾𝛿⁺ T-cell responders was compared between cohorts by a Chi-squared test using the statistical program SAS Enterprise Guide 4.3.

Cohort	No. of; subjects	𝛾𝛿^+ T-cell; responders		𝛾𝛿^+ T-cell; nonresponders
		𝑁	%	𝑁	%
A; (≥18 to ≤45 years)	186	25*	13.4	161	86.6
B; (≥60 to <75 years)	240	36*	15.0	204	85.0
C; (≥75 years)	79	11*	13.9	68	86.1
D; (≥60 years in LTCF)	105	18*	17.1	87	82.9
All	610	90	14.8	520	85.2

 ^∗𝑃=0.85.