Involvement of MicroRNA in Microglia-Mediated Immune Response
Figure 2
MiR-155 inhibition in microglia cells increases SOCS-1 levels and decreases the release of inflammatory cytokines to the extracellular medium. N9 mouse microglia cells were transfected with anti-miR-155 oligonucleotides (anti-miR155) or with nontargeting oligonucleotides (oligo scramble) complexed with cationic liposomes for 4 h. Twenty-four hours after transfection, cells were incubated with LPS at 0.1 μg/mL for 18 h. The cell culture medium was then collected to determine cytokine protein levels, and total RNA was extracted from the cultured cells. (a) SOCS-1 mRNA levels were quantified by qRT-PCR. (b) The levels of TNF-α, IL-1β, and IL-6 secreted to the cultured medium were determined by ELISA. Results are expressed as fold change of mRNA or protein levels with respect to control (untransfected and untreated cells). compared to cells transfected with the scramble oligonucleotide and compared to LPS-treated cells in the absence of transfection. Results in (a) and (b) are representative of 3 independent experiments performed in triplicate.
