Low-Dose Amphotericin B and Murine Dialyzable Spleen Extracts Protect against Systemic Candida Infection in Mice

Figure 1

Effect of low-dose AmB and mDSE on murine systemic candidiasis. (a) Mice were infected with the indicated amounts of Ca07-387 blastospores and were observed for 30 days. Data from two independent experiments were used. (b) Different concentrations of AmB were administered to mice that had received 5 × 10⁵ CFU of Ca07-387. (c) Mice that had received 5 × 10⁵ CFU of Ca07-387 were treated with 10 pg of mDSE, alone or in combination with 0.1 mg/kg of AmB. Data from two different experiments were used. (d) Kidney fungal burdens (CFU/g) each point represents mean and SD of three mice. In (d), the dotted line indicates the assay detection limit (<50 CFU) (**𝑃<0.01; ***𝑃<0.001, AmB versus AmB + mDSE), (𝛼=𝑃<0.001, mDSE versus Infected no Tx), (𝛽=𝑃<0.001, mDSE versus AmB), (𝛾=𝑃<0.001, mDSE versus AmB + mDSE), (𝛿=𝑃<0.05, Infected no Tx versus AmB), (𝜀=𝑃<0.05, Infected no Tx versus AmB + mDSE).

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