The Leech Nervous System: A Valuable Model to Study the Microglia Involvement in Regenerative Processes
Figure 5
Chemotactic effect of recombinant HmC1q on leech microglia in isolated and crushed segment of nerve cord maintained in tissue culture (see the diagram (c)). (a) Hoechst-dyed microglial cells strongly accumulated 4 hours after injection of rHmC1q (arrows). (b) When tissues have been injected with polyclonal anti-HmC1q antibody, the chemotactic activity of rHmC1q has not been observed (arrows). To note, the corresponding preimmune serum did not exert any neutralizing effect (data not shown). (Reprinted, with permission, from [74]).
