Prolactin Levels Correlate with Abnormal B Cell Maturation in MRL and MRL/lpr Mouse Models of Systemic Lupus Erythematosus-Like Disease
Figure 1
Purification of B cell by flow cytometry. B cells were purified from the BM of 9-week-old mice. (a) The cells were incubated with antibodies specific for B cell subsets, and the subsets were purified using flow cytometry, as detailed in Methods. The purity of the collected populations varied between 95% and 98%. A representative example of the purified B cells from wild-type C57BL/6 mice is shown. (b) Using RT-PCR, the PRL receptor mRNA expression was measured in the different subsets of bone marrow B cells (pro-B, pre-B and immature). The asterisks denote statistical significance with the value shown.
