Figure 3: Heat-killed Candida-(HKC-) induced IL-23 expression in human monocytes in cell cultures containing LPS. (a) LPS pretreated human PBMC with HKC challenge and expression of mRNA for IL-12p40 and IL-23p19 subunits of IL-23. A significantly increased IL-23 production in an LPS dose-dependent manner was evident compared to cells without LPS stimulation ( ). (b) Human monocytes cell line (THP-1) produced IL-23 in a dose-dependent manner in the cell cultures together with LPS. (c) Human PBMC produced IL-17A after days 1, 3, and 7 cultured with 3 different clinical strains of HKC.