Persistence of Th17/Tc17 Cell Expression upon Smoking Cessation in Mice with Cigarette Smoke-Induced Emphysema

Figure 3

Increased numbers of CD4⁺IL-17⁺ (Th17) cells and CD8⁺IL-17⁺ T (Tc17) cells are not reversible after smoking cessation.Lung single-cell from nonsmoke mice, smoke-exposed mice, and smoke cessation mice was stimulated by PMA and ionomycin for 4 h and analyzed IL-17A by using flow cytometry. (a) Representative FACS staining for IL-17A in gated CD4⁺ T cells. (b) Representative FACS staining for IL-17A in gated CD8⁺ T cells. (c) The frequencies (%) of Th17 cells; (d) the frequencies (%) of Tc17 cells. Results are expressed as % (mean ± SEM). 𝑛=10 animals/group; *𝑃<0.05.

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