Research Article

Investigation of Functional Activity of Cells in Granulomatous Inflammatory Lesions from Mice with Latent Tuberculous Infection in the New Ex Vivo Model

Figure 6

The macrophages (Mph), dendritic cells (DC), and lymphocytes (Lym) with production of (a) IFNγ, (b) IL-1α, (c) GM-CSF and (d) FGFb. The independently examined objects are control peritoneal macrophages (ConMph) from three intact mice, peritoneal macrophages obtained from mouse 25 (25Mph), that was intraperitoneally infected with the BCG vaccine for two months, cells in leukocyte infiltrates from mouse 6S/2 and granulomas from the spleens of three to six mice after one and two months (Gran/1 and Gran/2, resp.) of BCG infection in vivo and ex vivo culture after a few days. Data are means ± SEM. * (comparing cytokine-positive macrophages in controls (ConMph and 25Mph) and Gran/1 and Gran/2 granulomas), ** , *** , & .