Expression of B7-H1 on HSCs in response to INF-γ stimulation. (a) HSCs isolated from B6 mice were exposed to graded concentrations of IFN-γ (0.1–200 U/mL) for 24 hours in vitro and stained with anti-B7-H1 mAb and analyzed by flow cytometry. (b) HSCs were treated with IFN-γ (10 U/mL) for varying times (0.5–24 hours) and analyzed by RT-PCR. (c) HSCs were incubated with IFN-γ (100 U/mL) for the indicated times, and the expression patterns were analyzed by flow cytometry. (d) HSCs isolated from wild type (WT) or IFN-γR1 KO mice or Stat1 KO mice (all on B6 background) were exposed to IFN-γ (100 U/mL) for 48 hours. Cells were stained using anti-B7-H1 mAb and analyzed by flow cytometry. The data are representative of two separate experiments.
