The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
Figure 2
TNF-α induced receptor mobilization does not involve the PAFR. Human neutrophils (5 × 105) were incubated on ice (solid lines), or at 37°C without additive (dotted lines), or with TNF-α (10 ng/mL; dashed lines) for 20 min. The cells were then fixed with 2% ice-cold paraformaldehyde and labeled with specific antibodies against PAFR (A) or complement receptor 3 (CR3; B). Surface receptor expression was determined by flow cytometry, and a representative histogram is shown. Abscissa: fluorescence intensity; Ordinate: number of cells. The insets show the mean fluorescence intensity (MFI) ± SEM ().
