The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
Figure 3
PAF and fMLF dose dependently trigger the release of superoxide anions from human neutrophils. Human neutrophils (105 cells) were incubated at 37°C for 5 min in measuring vials containing isoluminol and HRP. Various concentrations of fMLF (A) or PAF (B) were added, and the release of superoxide anions was recorded continuously. Data are expressed as mean peak value ± SEM (). Abscissa, agonist concentration; ordinate, superoxide production expressed as counts per minute 106 (Mcpm). The insets show one representative normalized dose-response experiment with respective EC50 values calculated for fMLF (A) and PAF (B), respectively.
