The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration
Figure 3
Adhesive effect of Netrin-1 on thymocytes. (a) Quantification of thymocyte adhesion to plate coated with BSA, fibronectin (Fn), or Netrin-1. (b) The inserts of Transwell were coated with different concentrations of Netrin-1, and thymocytes were plated in the upper chamber of Transwell. Histograms represent the number of migrating thymocytes recovered in the lower chamber. (c) Thymocytes were incubated with the function-blocking anti-integrin antibody for 30 min prior to plating on Netrin-1. Cells were allowed to attach for 30 min, then washed, and quantified as described in Materials and Methods. (d) In the presence of Netrin-1 (500 ng/mL), thymocytes were plated in the upper chamber of Transwell under cell migration driven by fibronectin (10 g/mL). Histograms represent the number of migrating thymocytes in the lower chamber. Data shown in each image are representative of three independent experiments. ns: not significant; ; ; .
