The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration

Figure 4

Netrin-1 up-regulation of CXCL12-driven mice thymocyte migration. (a) Netrin-1 partially augmented the chemoattraction of mice thymocytes induced by CXCL12 in the lower chamber. (b) Cells migrated to the bottom well from (a) were harvested and stained with anti-CD4 and anti-CD8. The numbers in the panels are % of gated populations. (c) The effect of Netrin-1 on CXCL12 could be seen in total thymocytes, as well as in DN, DP, and CD8SP subsets. (d) Netrin-1 had no effect on total thymocytes migration induced by CCL19. (e) Cells migrated to the bottom well from (d) were harvested and stained with anti-CD4 and anti-CD8. The numbers in the panels are % of gated populations. Experiments are representatives of at least three independent experiments. ns: not significant; ^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001.

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