Figure 1: Construction of expression vector. (a) PCR products from three-round amplification. M, DNA marker; lane 1, GAPDH as internal control; lane 2, VH; lane 3, VL; lane 4, CH1; lane 5, CL; lane 6, heavy chain Fd; lane 7, light chain; lane 8, Fab. (b) Recombinant vector pComb3X/LF8-Fab recut by Sfi I. M, DNA marker; lane 1, pComb3XSS cut by Sfi I; lane 2, pComb3X/LF8-Fab recut by Sfi I; lane 3, PCR product of Fab.