Figure 3: Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and IRDye 800CW donkey antigoat IgG were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.