Effects of Muscarinic Acetylcholine 3 Receptor208-227 Peptide Immunization on Autoimmune Response in Nonobese Diabetic Mice
Figure 5
Lymphocytic infiltration was improved in the salivary glands following immunization with the M3R208–227 peptide. Whole salivary glands of NOD/LtJ mice were surgically removed from each mouse and placed in 10% phosphate-buffered formalin for 20 min. After the samples were fixed, embedded, and stained with hematoxylin and eosin (H&E), sections were observed under a microscope. The histological analysis suggested that immunization with M3R208–227 could reduce the lymphocytic infiltration in the salivary glands of NOD/LtJ mice. (a) Tissue from a NOD/LtJ mouse immunized with the M3R208–227 peptide. (b) Tissue from a NOD/LtJ mouse immunized with CP. (c) Tissue from a NOD/LtJ mouse immunized with PBS. (d) Mean grade (histological score) of inflammatory lesions in salivary glands of NOD/LtJ mouse immunized with the M3R208–227 peptide as compared to the controls ().
