Microglia Play a Major Role in Direct Viral-Induced Demyelination
Figure 1
Colocalization of EGFP-expressing cells (positive for viral antigen) and microglia/macrophages (CD11b) in spinal cord white matter (dorsal columns) of RSA59-infected mice. RSA59-infected mice (low magnification (a–c); high magnification (d–f)). EGFP fluorescence (green) identifies virus-infected cells (a, d) and anti-CD11b (red fluorescence) detects microglia/macrophages (b, e). Merged images (c, f) in RSA59-infected spinal cord show colocalization of EGFP in CD11b-positive cells in spinal cord white matter. Arrows mark CD11b-positive cells that are infected (EGFP-positive), whereas arrowheads mark CD11b-positive cells that are not infected (EGFP-negative).
