Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis

Figure 2

Autoantibodies against SLA detected with ELISA based on bacterially expressed protein. Bacterially expressed SLA (SLA-His-coli) was used to form the solid phase in an indirect ELISA for the determination of human IgG antibodies in 20 anti-SLA positive autoimmune hepatitis sera, 20 anti-SLA negative autoimmune hepatitis sera, 15 primary biliary cirrhosis sera, 15 HCV sera, and 30 sera from healthy blood donors. Positive and total numbers of sera are given below the diagrams. The cut-off value is presented by a dotted line.