Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant
Blood glucose levels in individual animals of all experimental groups. (a) Mock-infected experimental group of 5-week-old NOD mice () was inoculated with PBS. (b), (c), and (d) Three experimental groups of mice () were subjected to s.c. injection with 0.1 mL of undiluted (1 : 0) and 1 : 10 and 1 : 100 diluted CFA. (e), (f), and (g) Groups of mice rVV-CTB::GAD + CFA (1 : 0), and rVV-CTB::GAD + CFA (1 : 10), rVV-CTB::GAD + CFA (1 : 100) () were injected with the 0.3 mL virus (5 × 107 PFU/mL) i.p. and s.c. coinoculated with 0.1 mL CFA. (h) The rVV-CTB::GAD group of mice () was subjected to i.p. injection with 0.3 mL of the virus (5 × 107 PFU/mL). Two weeks after the first injection, the vaccine inoculations were repeated. Beginning at 13 wks of age the individual mice in each experimental group were bled from the tail vein biweekly for 119 wks, and blood sugar levels were quantified.