Overexpression of HIF-2α, TWIST, and CXCR4 Is Associated with Lymph Node Metastasis in Papillary Thyroid Carcinoma

Table 1

Primers used for real-time RT-PCR.

Gene	Primers	Product size
HIF-2𝛼	Forward: 5′-TCTGAAAACGAGTCCGAAGCC-3′	196 bp
	Reverse: 5′-GGTCGCAGGGATGAGTGAAGT-3′
TWIST	Forward: 5′-CGACGACAGCCTGAGCAACA-3′	207 bp
	Reverse: 5′-CCACAGCCCGCAGACTTCTT-3′
CXCR4	Forward: 5′-CCACGCCACCAACAGTCAGA-3′	109 bp
	Reverse: 5′-GGCAAAGATGAAGTCGGGAATA-3′
GAPDH	Forward: 5′-GGAGTCCACTGGCGTCTTCA-3′	191 bp
	Reverse: 5′-GGGGTGCTAAGCAGTTGGTG-3′