Comparing Effects of BK Virus Agnoprotein and Herpes Simplex-1 ICP47 on MHC-I and MHC-II Expression

Figure 2

(A) HLA-ABC and -DR surface expression in UTA-6 cells transiently transfected with ICP47. HLA-ABC and -DR surface expression was analyzed by flow cytometry of EGFP-gated UTA-6 cells, cotransfected with pEGFP-N1 and pTRE-ICP47, and cultured for 48 hours with different concentration of IFNγ, with (□) or without (♦) tetracycline. MFI: mean fluorescence intensity. (B) HLA-ABC and -DR surface expression in UTA-6 cells transiently transfected with agnoprotein. HLA-ABC and -DR surface expression was analyzed by flow cytometry of EGFP-gated UTA-6 cells, cotransfected with pEGFP-N1 and phTRE-agno, and cultured for 48 hours with different concentration of IFNγ, with (□) or without (♦) tetracycline. MFI: mean fluorescence intensity.