Trichostatin A Promotes the Generation and Suppressive Functions of Regulatory T Cells

Figure 2

TSA increases the generation of Tregs from naive CD4⁺ T cells. (a) Flow cytometry analysis of CD4⁺Foxp3⁺ cells generated in the absence (left) or presence (right) of 10 nM TSA for 4 days. Numbers indicate the percentage of CD4⁺Foxp3⁺ double positive cells. (b) Scatter plot depicts percentage of CD4⁺Foxp3⁺ double positive cells from five independent experiments in the presence and absence of TSA. ^∗∗𝑃<0.008 by student’s 𝑡-tests. (c) Western blot analyses of total protein extracts derived from Tregs differentiated in the absence or presence of 10 nM TSA, as indicated. (d) Quantitation of the acetylation levels relative to the histone H3 with the Quantity One software. Standard deviation was obtained from three independent experiments. ^∗𝑃<0.05 by two-way ANOVA.

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