Trichostatin A Promotes the Generation and Suppressive Functions of Regulatory T Cells
Figure 3
TSA Treg presents higher suppressive capacity. (a) Treg suppression assays of Tregs generated in the presence (left) or absence (right) of dendritic cells, and with (+TSA) or without TSA (−TSA). Tregs from each group were added to CFSE-labeled naive CD4+ T cells cocultivated with dendritic cells plus anti-CD3 antibody. The percentage of proliferating T cells is shown in each plot. Data are representative of three independent experiments. (b) Quantitation of the percentage of suppression of Tregs generated with or without TSA in the presence of anti-CD3 and dendritic cells (left) or plate-bound anti-CD3 and soluble anti-CD28 (right).
