BCG and BCG/DNAhsp65 Vaccinations Promote Protective Effects without Deleterious Consequences for Experimental Autoimmune Encephalomyelitis
Figure 4
Effect of tuberculosis vaccines in IFN-γ and IL-10 production. Female Lewis rats were immunized with BCG alone or with BCG followed by pVAXhsp65 boosters and then underwent EAE induction by inoculation of MBP emulsified with CFA. Animals were euthanized during the recovery phase (20th day after MBP inoculation) and spleen and lymph node cell cultures were stimulated with rhsp65 ((a) and (d)); MBP ((b) and (e)); and ConA ((c) and (f)). IFN-γ levels were evaluated in spleen cell cultures ((a), (b), and (c)) and IL-10 levels were evaluated in lymph node cell cultures ((d), (e), and (f)). Data are presented by mean ± SEM for 4–6 animals. *Represents the difference between immunized and control groups. .
