Increased Frequency of Bone Marrow T Follicular Helper Cells in Patients with Immune-Related Pancytopenia
Figure 3
Summary of FACS analysis of BM follicular helper T (Tfh) cells from 90 IRP patients and 25 controls. (a) Tfh cells are expressed as a percentage of CD4+ lymphocytes. Each data point represents an individual subject; horizontal lines show the median. Rhombus indicate untreated IRP patients, triangles indicate recovered IRP patients and circles indicate controls. (b) Comparison of ICOS and CD40L expression according to the expression of CXCR5 on CD4+ cells in IRP patients and healthy controls. On the left -axis, circles indicate CXCR5+ICOS+ cells among the CD4 lymphocytes; on the right -axis, squares indicate CXCR5+CD40L+ cells among the CD4 lymphocytes. Each data point represents an individual subject; horizontal lines show the median. Red indicates untreated IRP patients, blue indicates recovered IRP patients and black indicates controls. (c) Percentage of intracytoplasmic IL-21+CXCR5+ in CD4 cells in IRP patients, and controls. Each data point represents an individual subject; horizontal lines show the median. Rhombus indicate untreated IRP patients, triangles indicates recovered IRP patients and circles indicate controls. (d) Comparison of Tfh cells, IL-21, ICOS, and CD40L expression, according to the expression of CXCR5 on CD4+ cells in IRP patients, and healthy controls. Data correspond to the mean ± SD. (e) Detection of Bcl-6 mRNA expression in IRP patients and healthy controls. Bcl-6 mRNA was estimated by real-time RT-PCR. Data correspond to the mean ± SD.
