Research Article

Hepatic Expression of Metallothionein I/II, Glycoprotein 96, IL-6, and TGF-β in Rat Strains with Different Susceptibilities to Experimental Autoimmune Encephalomyelitis

Figure 2

Expression of gp96 in the livers of EAE-prone DA and EAE-resistant AO rats. (a) Representative western blots indicate the gp96/GRP94 protein (~200 kD; range 150–200 kD), detected by MoAb to Grp94/gp96 (clone 9G10) under a nonreducing condition in SDS-PAGE. Liver samples were obtained from intact rats and from rats treated by bovine brain homogenate (BBH) and complete Freund’s adjuvant (CFA) or by CFA at the time of first attack in EAE-prone rats (day 12). As a loading control the blots of β-actin protein (37–50 kD) are shown. (b) Relative protein expression is shown as AO/DA ratio (intact liver) and as the percent of control (liver in EAE). All band densities were normalized corresponding to -actin. Analyses were made in tissue samples, prepared as a pool from 3 rats in three independent experiments. Data represent mean ± SE. (c) Representative immunohistochemical staining of gp96 protein in paraffin-embedded sections of the liver tissue in DA and AO rats after treatment with BBH+CFA or CFA. The results are representative findings of 3 rats.