What Are the Molecules Involved in Regulatory T-Cells Induction by Dendritic Cells in Cancer?

Figure 2

Patients’ Mo-DCs fail to activate CD4⁺ lymphocytes and induce higher Foxp3 expression even after maturation. Mo-DCs from controls and breast cancer patients were cocultured with allogeneic CD4⁺CD45RA⁺ cells for five days. At the end of culture the phenotype of lymphocytes was evaluated by flow cytometry. (a) Representative experiments of CD25 and Foxp3 expression in CD4⁺ lymphocytes stimulated by immature DCs (Mo-iDCs) or mature DCs (Mo-mDCs) from healthy donors or breast cancer patients. Average frequency of CD25⁺ cells (b) and CD4⁺CD25⁺Foxp3⁺ cells (c) after CD4⁺CD45RA⁺ lymphocytes’ coculture with Mo-DCs (*𝑃<0.05;  **𝑃<0.01, two-tailed unpaired t-test; 𝑛=4). (Mature Mo-DCs were activated by TNF-alfa for 48 hours.)

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