A New ELISA for Dermatomyositis Autoantibodies: Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement

<table>ELISA development using biotinylated recombinant protein. The process flow summarizes the method of ELISA construction, from obtaining the cDNA to obtaining the data by luminometer. We perform phenol/chloroform treatments twice to inhibit RNase for the plasmid purification.</table>

Journal of Immunology Research

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Figure 1

Figure 1: A New ELISA for Dermatomyositis Autoantibodies: Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement 