Journal of Immunology Research / 2013 / Article / Fig 2

Research Article

An Imbalance between Frequency of CD4+CD25+FOXP3+ Regulatory T Cells and CCR4+ and CCR9+ Circulating Helper T Cells Is Associated with Active Perennial Allergic Conjunctivitis

Figure 2

Frequency of chemokine receptors positive cells on circulating PBMC from healthy controls (HC) and from patients with perennial allergic conjunctivitis (PAC) PBMC were stained with fluorescence-conjugated antibodies to CD4 and (a) CCR4, (b) CCR7, and (c) CCR9, in a double-immunofluorescence assay, as described in materials and methods. Representative histograms of CD4+ gated cells are shown (d) CD4+CCR4+, (e) CD4+CCR7+, and (f) CD4+CCR9+ cells in both, HC and patients with PAC. Representative dot plots and histograms from 7 HC and 14 PAC.
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