LPS-Induced Inflammation Potentiates the IL-1𝜷-Mediated Reduction of LH Secretion from the Anterior Pituitary Explants

Figure 1

LH release from the AP explants collected from saline- and LPS- treated ewes and incubated in four types of media (control—“pure” medium 199 HEPES (a); GnRH control—medium with GnRH (100 pmol/mL) (b); IL-1β—medium with IL-β (100 pg/mL) (c); GnRH + IL-1β medium with GnRH (100 pmol/mL) and IL-β (100 pg/mL) (d)). Each curve represents median ± SEM release of LH during the consecutive 20 min periods of incubation. ^†𝑃≤0.01 (cross indicates values that differ significantly from the median LH release in the same group during preincubation period according to the Mann-Whitney 𝑈 test) ^∗𝑃≤0.01 (asterisk indicates values that differ significantly from the saline- treated group according to the Mann-Whitney 𝑈 test).

(a)

(b)

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(d)