Figure 3: Influence of the culture medium on the efficiency of peptide stimulation. 107 PBMC from HLA-A2 healthy donors (a and b) or from melanoma patients (c) were stimulated in 96-well plated (2 × 105 cells/well) with either Melan- peptide (a) at 1 μM or MELOE-136-44 peptide (b and c) at 10 μM, in either RPMI 8% HS or X-Vivo 15 in the presence of 50 IU/mL of IL-2. (a) Percentages of Melan-A- or (b and c) MELOE-1-specific T cells detected by double labelling with tetramer and anti-CD8 antibody, in microcultures stimulated in the two culture media. Each symbol corresponds to a microculture containing more than 0.5% of specific T cells.