Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain
Figure 2
PC61 treatment of glioma-bearing mice leads to local infiltration with macrophages and granulocytes. (a) Flowcytometric phenotyping of brain-infiltrating myeloid cells in control (upper row) and PC61-treated mice (lower row). Based on the expression of CD45 and CD11b, three populations of brain-infiltrating cells were identified: CD45+CD11b− lymphocytes (in red), CD45+CD11b+ myeloid cells (in green), and CD45-CD11b+ microglial cells (in blue). Within the CD45+CD11b+ myeloid cells, expression of Ly6G (Gr-1) and F4/80 was measured. (b) Morphologic analysis of brain-infiltrating myeloid cells on cytospins. Detail from a representative FOV (40x) from control IgG-treated mice (left) and mice that were PC61 treated (right). Macrophages (black arrows) and granulocytes (white arrows) are highlighted.
