T Cell Vaccination Inhibits Th1/Th17/Tfh Frequencies and Production of Autoantibodies in Collagen-Induced Arthritis
TCV influenced the frequency of Th1/Th17 cells and related cytokines in CIA mice. The three groups of mice were sacrificed at the peak of the disease. DLN MNCs were harvested. IFN-γ and IL-17 secreting cells were determined by intracellular staining and flow cytometry. Results showed the percentage of Th1/Th17 cells in DLN CD4+ cells (a)-(b). (c) Results showed the absolute number of Th1/Th17 cells in DLN. (d) Heart blood was collected and the serum was isolated by centrifuge. DLN MNCs were prepared and incubated (5 × 105 cells/well) in the absence and presence of heat-inactivated CII (20 μg/mL) for 48 h and the supernatants were collected. The levels of IFN-γ and IL-17 were determined using ELISA. Concentration of IFN-γ and IL-17 in sera (left) and supernatants of cell culture (right). Data are represented as means ± SD ( mice/group). Data are representative of 5 separate experiments with similar results. *, **, CIA versus normal group. #, TCV versus normal group. , TCV versus CIA group.
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