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Clinical and Developmental Immunology
Volume 2013, Article ID 978202, 10 pages
http://dx.doi.org/10.1155/2013/978202
Research Article

Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B

1Immunology and Allergology Laboratory Unit, Azienda Sanitaria di Firenze, San Giovanni di Dio Hospital, Florence, Italy
2Department of Research, INOVA Diagnostics Inc., 9900 Old Grove Road, San Diego, CA 92131-1638, USA
3Rheumatology Unit, Department of Internal Medicine, San Giovanni di Dio Hospital, Azienda Sanitaria di Firenze, Florence, Italy
4Rheumatology Unit, L. Sacco University Hospital, Milan, Italy

Received 23 July 2013; Accepted 26 September 2013

Academic Editor: Marvin J. Fritzler

Copyright © 2013 M. Infantino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B. Methods. The study included 241 samples, from patients suffering from systemic autoimmune diseases ( ) as well as disease controls ( ). All samples were tested for anti-Ro52, anti-Ro60, and anti-SS-B (La) antibodies on QUANTA Flash (INOVA Diagnostics, San Diego, USA) and BioPlex 2200 (Bio-Rad Laboratories Inc., Hercules, USA). Discrepant samples were tested by two independent methods: BlueDot/ANA and QUANTRIX Microarray (both D-tek, Belgium). Results. The overall qualitative agreements were 95.4% (95% confidence interval, CI 92.0–97.7%) for anti-Ro52, 98.8% (95% CI 96.4–99.7%) for anti-Ro60, and 91.7% (95% CI 87.5–94.9%) for anti-SS-B antibodies. There were 34 discrepant samples among all assays (20 anti-SS-B, 11 anti-Ro52, 3 anti-Ro60). 30/33 of retested samples (by D-tek dot blot) agreed with the QUANTA Flash results. Similar findings were obtained with QUANTRIX Microarray kit. Conclusion. QUANTA Flash and BioPlex 2200 show good qualitative agreement. The clinical performances were similar for anti-Ro52 and anti-Ro60 autoantibodies while differences were observed for anti-SS-B (La) antibodies.