Interpretation of ANA Indirect Immunofluorescence Test Outside the Darkroom Using NOVA View Compared to Manual Microscopy

Table 1

NOVA View agreement with manual IIF: 1 : 40 screening dilution.

All patients (𝑛=369)	Manual IIF			Percent agreement (95% confidence)
	Positive	Negative	Total
NOVA View
Positive	128	6	134	Positive agreement = 94.8% (89.6–97.9%)
Negative	7	228	235	Negative agreement = 97.4% (94.5–99.1%)
Total	135	234	369	Total agreement = 96.5% (94.1–98.1%)
				𝜅 = 0.92 (95% CI 0.88–0.96)

𝑁: number of patients tested at a 1 : 40 dilution for HEp-2 ANA antibodies.
Manual results were read on a Nikon Eclipse 400 fluorescent microscope with an LED light source.
NOVA View confirmed results = archived images reviewed and confirmed by the operator.