HLA-G Dimers in the Prolongation of Kidney Allograft Survival
Figure 2
Increased percentages of HLA-G-positive monocytes in nonrejecting kidney transplant patients. (a, b, c) PBMCs from renal allograft patients in both the nonrejected group (NR) and chronic rejected (CR) groups were stained with anti-CD3, anti-CD14, and anti-HLA-G mAbs, and FACS analysis was performed. Representative flow cytometric analysis of PBMCs from indicated groups of renal allograft recipients is shown. (b and c) Histograms shown here were gated on a CD14-positive population. Filled histograms represent the isotype control. Numbers indicate the percentage of HLA-G-positive monocytes. (d) Numbers indicate the percentage of HLA-G-positive T cells in both groups of patients (gated on CD3-positive population). Filled boxes represent data from NR and open boxes represent data from CR patients. (e) Numbers indicate the percentage of HLA-G-positive monocytes in both groups of patients (gated on CD14-positive population). Data are shown as mean ± SD and analyzed by Student’s -test. NS: not significant.