Regulatory Lymphocytes Are Key Factors in MHC-Independent Resistance to EAE
Figure 2
Effector and regulatory B cells in spleens of C57BL/6 and CD1 mice. We analyzed these cells in series of seven mice per condition. (a) B2 cells (B220+CD5−) did not change significantly in mice immunized with MOG35–55 (MOG) or PLP139–151 (PLP) when compared with nonimmunized (NI) ones. (b) B1 cell (B220+CD5+) percentage diminished in C57BL/6 mice that developed EAE upon immunization with MOG35–55. (c) The B220+CD5+CD1dhi regulatory subset (Breg) increased in resistant CD1 mice immunized with MOG. Conversely, it decreased in susceptible C57BL/6 mice developing EAE after immunization with the same peptide. (d) Representative dot plots showing Breg induced in CD1 (left side) and C57BL/6 (right side) mice upon immunization with MOG35–55 peptide. Total B cells were gated by selecting B-220 positive cells and then Breg were identified by CD5 and CD1dhi staining.
