GK-1 Improves the Immune Response Induced by Bone Marrow Dendritic Cells Loaded with MAGE-AX in Mice with Melanoma

Figure 1

BMDCs phenotype. Levels of molecules of the major histocompatibility complex II (MHCII), CD40, CD80, CD86, and IL-12 in BMDCs were measured after different treatments: control (without treatment: WT), LPS, GK-1, TNFα, TNFα/GK-1. Treatment with LPS, TNFα, and TNFα/GK-1 induces increased expression of MHCII, CD40, CD80, and CD86. When BMDCs were treated only with GK-1 an increase in the production of IL-12 was found. (a) Mean fluorescence intensity (MIF) of MHCII. , . (b) BMDCs phenotype after 10 days of differentiation. 91.9% differentiation was induced (91.9% of CD11c+ cells). Red: isotype control. Blue: BMDCs. (c) Percentage of CD40+ BMDCs after treatment. . (d). MFI of CD40 in BMDCs. , . (e) Percentage of CD86+ BMDCs. , . (f) MFI of CD86 in BMDCs. , , TNFα versus GK-1. (g) Percentage of CD80+ BMDCs. . (h) MFI of CD80 in BMDCs. ANOVA, Tukey. , TNF/GK-1 versus GK-1, TNF versus GK-1. (i) Percentage of IL-12+ BMDCs. (j) MFI of IL-12 in BMDCs. , , . Mean ± SEM .

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