Generation of Recombinant Schmallenberg Virus Nucleocapsid Protein in Yeast and Development of Virus-Specific Monoclonal Antibodies
Figure 1
Analysis of yeast cell lysates and purified SBV N protein by SDS-PAGE (a) and western blot (b). Purified SBV N protein (lane 3) or 20 μg of yeast lysates (lanes 1 and 2) was separated in a 12% SDS-PAGE gel and stained with Coomassie Brilliant Blue. PageRuler Unstained Protein Ladder (Thermo Fisher Scientific Baltics) was used. Lane 1, lysate of mock-transformed S. cerevisiae [pFX7-6-His]; lane 2, lysate of S. cerevisiae transformed with a plasmid [pFX7-6-His-N] encoding SBV N protein (the arrow indicates SBV N protein band); lane 3, Ni-chelate resin-purified SBV N protein. Western blotting was performed using the MAb against 6-His-tag epitope (b) (Thermo Scientific). PageRuler Prestained Protein Ladder (Thermo Fisher Scientific Baltics) was used.
