Generation of Recombinant Schmallenberg Virus Nucleocapsid Protein in Yeast and Development of Virus-Specific Monoclonal Antibodies

<table>SDS-PAGE (a) and western blot analysis of recombinant SBV N protein with SBV N-specific MAbs ((b)–(e)). Lane M, PageRuler Prestained Protein Ladder (Thermo Fisher Scientific Baltics); lane 1, SBV N protein; lane 2, Puumala N 6 His-tagged protein; lane 3, yeast cell lysate. Undiluted hybridoma culture supernatants were used. MAbs codes are indicated at the bottom of each picture.</table>

Journal of Immunology Research

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Figure 3

Figure 3: Generation of Recombinant Schmallenberg Virus Nucleocapsid Protein in Yeast and Development of Virus-Specific Monoclonal Antibodies 