Novel Clinical and Diagnostic Aspects of Antineutrophil Cytoplasmic Antibodies

Figure 4

Principle of QUANTA-Flash chemiluminescent immunoassays. Paramagnetic beads are coupled with native PR3 or MPO. The beads are then incubated with diluted patient samples. After 9.5 min incubation unbound antibodies are removed by washing. Antihuman IgG isoluminol conjugate (Tracer) is added and binds immobilized antibodies. After another 9.5 min incubation unbound Tracer is removed by washing. Finally, Trigger 1 and Trigger 2 are injected and emerging light is measured. After injection of Trigger 1 and Trigger 2, the luminescence is measured as relative luminescence units (RLU) [17].