Table 1: β2-Glycoprotein I antigen isolation procedure: comparison of standard and optimized protocols.

Standard protocolOptimized protocol 2GPI isolation from the same starting material after both protocols

AB plasma donors ( )1462
Number of isolations1251 (isolation I)1 (isolation II)
Starting volume, mean ± SD (mL) 250255
Number of aliquots1313
Precipitation duration, mean ± SD (min) 61.2521.25
Mass of isolated 2GPI, mean ± SD (mg) 7.2210.21
Efficiency improvement51.5%41.4%

2GPI, 2-glycoprotein I. Significantly higher mass as compared to standard protocol (Student’s -test, two-tail).