Research Article
Optimization of Unnicked β2-Glycoprotein I and High Avidity Anti-β2-Glycoprotein I Antibodies Isolation
Table 1
β2-Glycoprotein I antigen isolation procedure: comparison of standard and optimized protocols.
| | | Standard protocol | Optimized protocol | 2GPI isolation from the same starting material
after both protocols |
| | AB plasma donors () | 14 | 6 | 2 | | Number of isolations | 12 | 5 | 1 (isolation I) | 1 (isolation II) | | Starting volume, mean ± SD (mL) | | | 250 | 255 | | Number of aliquots | 1 | 3 | 1 | 3 | | Precipitation duration, mean ± SD (min) | | | 61.25 | 21.25 | | Mass of isolated 2GPI, mean ± SD (mg) | | | 7.22 | 10.21 | | Efficiency improvement | 51.5% | 41.4% |
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2GPI, 2-glycoprotein I. Significantly higher mass as compared to standard protocol (Student’s -test, two-tail).
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