Osteodifferentiated Mesenchymal Stem Cells from Bone Marrow and Adipose Tissue Express HLA-G and Display Immunomodulatory Properties in HLA-Mismatched Settings: Implications in Bone Repair Therapy
Figure 3
BM-derived MSCs committed to osteodifferentiation process express HLA-G and are hypoimmunogenic. (a and b) Expression of ALP and HLA-G5 was evaluated by intracellular flow cytometry analysis on BM-derived MSCs committed to 14-day osteodifferentiation process and pretreated with IFNγ and TNFα (Os-MSC-BM IFNγ/TNFα) or not (Os-MSC-BM). IgG1 was used as isotype control Ab. Tubulin was used as positive control of cell permeabilization. (c) PBMC from healthy individual (#00) were used as responder cells towards BM-derived MSCs committed to osteodifferentiation process and pretreated with IFNγ and TNFα (Os-MSC-BM IFNγ/TNFα) or not (Os-MSC-BM) as stimulating cells at various responder: stimulator ratios. Irradiated were used as positive control of T cell alloproliferation. Results are given as mean cpm ± s.e.m.; one representative experiment is shown.
