Osteodifferentiated Mesenchymal Stem Cells from Bone Marrow and Adipose Tissue Express HLA-G and Display Immunomodulatory Properties in HLA-Mismatched Settings: Implications in Bone Repair Therapy

Table 3

Immunogenicity of MSC from bone marrow and adipose tissue combined with MBCP biomaterial and licensing by IFN-γ and TNF-α.

	MSC-BM	MSC-BM; IFN-γ/TNF-α	MSC-AT	MSC-AT; IFN-γ/TNF-α
No MBCP^a	7.5±2.2^b	9.8±4.0	13.9±3.9	23.0±5.2
MBCP	51.9±14.1	41.1±11.6	8.1±0.8	11.0±0.8

aPBMC from healthy individuals were used as responder cells towards BM- or AT-derived MSCs pretreated with IFN-γ and TNF-α or not as stimulating cells at 1 : 0.2 responder : stimulator ratio.
^bData are mean ± s.e.m. of alloproliferation percentage obtained with 3 distinct healthy donors. This percentage is calculated considering PBMC stimulated with LCL* as 100% alloproliferation.