A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies
Figure 3
Calcium signaling upon agonist ACh stimulation of the aLC explant-cultured cells (a). The traces (b) represent the time courses of the 360/380 ratio , proportional to and correspond to the regions of interest (ROI) shown on the B&W image (a) in the same colors (explant growth time: 28 days).